Methods Testing

Weather conspired against us today! The roads to Salar de Atacama and El Tatio were both closed due to heavy and unusual weather, rainfall that turned into snow at one point during the morning. We made use of our time by performing extractions on a test sample using Purelyse, Omnilyse, Terralyzer and a Quick DNA kit (tissue version) to determine which would be most successful in the field. We tested the success of our extractions using the Qubit, and we determined that our most successful method was the Terralyzer. Guess it will be joining us in the field! We also simulated a field extraction and sequencing using a Zymo community standard and the RAD002 sequencing library on the MinION.